West Nile Virus Ns3 Protease: Insights into Substrate Binding and Processing through Combined Modelling, Protease Mutagenesis and Kinetic Studies
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WEST NILE VIRUS NS3 PROTEASE: INSIGHTS INTO SUBSTRATE BINDING AND PROCESSING THROUGH COMBINED MODELLING, PROTEASE MUTAGENESIS AND KINETIC STUDIES. Keith J. Chappell#, Martin J. Stoermer#, David P. Fairlie, and Paul R. Young From the School of Molecular and Microbial Sciences and Centre for Drug Design and Development, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia. Running title: Substrate processing by the West Nile virus protease Address correspondence to: Paul R Young, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia 4072, Tel. 617 33654646; Fax. 617 3365 4620; E-Mail: [email protected] and/or David P. Fairlie, Centre for Drug Design and Development, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia, 4072, Tel. 617 3346 2989; Fax. 617 3346 2101; E-Mail: [email protected] # These authors contributed equally to this work.
منابع مشابه
Site-directed mutagenesis and kinetic studies of the West Nile Virus NS3 protease identify key enzyme-substrate interactions.
The flavivirus West Nile virus (WNV) has spread rapidly throughout the world in recent years causing fever, meningitis, encephalitis, and fatalities. Because the viral protease NS2B/NS3 is essential for replication, it is attracting attention as a potential therapeutic target, although there are currently no antiviral inhibitors for any flavivirus. This paper focuses on elucidating interactions...
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West Nile Virus (WNV) is a mosquito-borne flavivirus with a rapidly expanding global distribution. Infection causes severe neurological disease and fatalities in both human and animal hosts. The West Nile viral protease (NS2B-NS3) is essential for post-translational processing in host-infected cells of a viral polypeptide precursor into structural and functional viral proteins, and its inhibiti...
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تاریخ انتشار 2006